Session Information
Date: Wednesday, June 7, 2017
Session Title: Neuropharmacology
Session Time: 1:15pm-2:45pm
Location: Exhibit Hall C
Objective: To explore the effect of apocyanin on glia mediated inflammatory response and α-synuclein aggregation in single intranigral LPS induced PD model
Background: Converging lines of evidence suggest that glia associated neuroinflammatory processes may account for the progressive death of dopaminergic neurons in Parkinson’s disease (PD). Also, Apocyanin, an established microglial NADPH oxidase inhibitor has been proved to have beneficial effects in modulating anti-inflammatory as well as anti-oxidative effects in case of lipopolysaccharide induced PD model.
Methods: LPS (5µg/kg b.wt) was injected intranigral stereotaxically and apocyanin (10mg/kg/ day) was given i.p. for a period of 21 days.
Results: Following LPS injection significant augmentation in the gene as well as protein expression of transcription factor NFκB as well as proinflammatory cytokines (TNF-α, IL-1α, IL-1β), NADPH oxidase subunits gp90PHOX and gp21PHOX were observed in the glial fraction thus suggesting the prevalence of inflammatory responses and activation of NADPH oxidase complex. IHC for microglial activation and α-synuclein revealed that apocyanin significantly inhibited microglial activation as well as alpha synuclein aggregation in dopaminergic cells. With this significantly compromised glutathione system as well as other antioxidant enzymes (SOD,Catalase) were also observed. However, with apocyanin treatment marked improvement in NFκB activation and related parameters was observed. This was further reflected in histopathological studies showing no evidence of inflammation in case of apocyanin treated animals
Conclusions: This can be concluded that apocyanin play an important role in modulating glial cell functions thus revealing its potential anti-inflammatory role along with its NADPH oxidase inhibiting property. Therefore, its neuroprotective role could be further evaluated in other toxicological conditions.
To cite this abstract in AMA style:
N. Sharma, B. Nehru. Role of Apocyanin in modulating glial cell functions and associated inflammatory response in Lipopolysaccharide induced Parkinson’s disease model. [abstract]. Mov Disord. 2017; 32 (suppl 2). https://www.mdsabstracts.org/abstract/role-of-apocyanin-in-modulating-glial-cell-functions-and-associated-inflammatory-response-in-lipopolysaccharide-induced-parkinsons-disease-model/. Accessed May 11, 2025.« Back to 2017 International Congress
MDS Abstracts - https://www.mdsabstracts.org/abstract/role-of-apocyanin-in-modulating-glial-cell-functions-and-associated-inflammatory-response-in-lipopolysaccharide-induced-parkinsons-disease-model/