Objective: We examined the relationship between intestinal bacteria, oral bacteria, bacterial metabolites, residual pesticides and clinical symptoms.

Background: Intestinal environment is thought to be involved in the onset of PD. It is pointed out that changes in the intestinal bacterial flora, in particular, have a great influence on the cause and the degree of progression of pathological conditions including other diseases. Last year, we reported a meta analysis of intestinal bacteria including self cases. In that report, the intestinal flora of the world was greatly different in America, Europe and Asia. Asia has less incidence of PD. The difference between intestinal bacteria in PD patients and healthy subjects（HC） in Asia is likely to develop stronger PD.

Method: 87 elderly HC, 143 PD patients, 22 patients with REM sleep behavior, and 22 patients with DLB were examined. The compositions of intestinal bacteria and oral bacteria were analyzed using 16 s rRNA. In selected PD patients and HC, Metagenomic analysis was performed using illumina hiseq 2000, and the obtained data was assembling using megahit to perform functional analysis. Short chain fatty acid composition and pH in feces were also measured. The influence of remaining pesticide in the urine was also analyzed.

Results: There was no clear difference in α diversity and β diversity between HC and disease. In PD patients, Faecalibacterium and Blautia were decreased. Akkermancia was increased. In the functional analysis using Picrust, the function related to short-chain fatty acid metabolism was reduced. Metagenomic function analysis was almost same results. Short chain fatty acids in feces decreased and branched chain fatty acids increased. As the severity increased, butyric acid bacteria decreased. The pH in feces increased. Some salivary flora correlated with the composition of butyric acid bacteria. The amount of residual pesticide in urine was increased in patients with PD.

Conclusion: Production of short chain fatty acids and branched chain fatty acids are produced by intestinal bacteria. The results agreed with the change in the product. In addition, while HC is living with PD patients, residual agricultural chemicals in the HC urine is lower than PD. It was considered possible that it might be related to absorption in the intestine.

References: 1. Hasegawa S, Goto S, Tsuji H, Okuno T, Asahara T, Nomoto K, Shibata A, Fujisawa Y, Minato T, Okamoto A, Ohno K, Hirayama M. Intestinal Dysbiosis and Lowered Serum Lipopolysaccharide-Binding Protein in Parkinson’s Disease. PLoS One 2015, 10: e0142164. 2. Minato T, Maeda T, Fujisawa Y, Tsuji H, Nomoto K, Ohno K, Hirayama M. Progression of Parkinson’s disease is associated with gut dysbiosis: Two-year follow-up study. PLoS One 2017, 12: e0187307. 3. Abe K, Hirayama M, Ohno K, Shimamura T. An Enterotype-like Unigram Class Model for Identifying Microbial Associations with Diseases. BMC Bioinformatics 2018, 4. Abe K, Hirayama M, Ohno K, Shimamura T. A latent allocation model for the analysis of microbial composition and disease. BMC Bioinformatics 2018, 19: 519.

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MDS Abstracts - https://www.mdsabstracts.org/abstract/relationship-between-intestinal-bacteria-bacterial-metabolites-and-residual-pesticides-in-parkinsons-disease/