Objective: To reveal the effect of probiotic on the content and condition of intestinal microbiota in Parkinson’s disease (PD).

Background: Constipation is an important non-motor symptom in PD.

Methods: 30 subjects with PD in 1,0-3,0 Hoehn-Yahr stage, 12 men and 18 women, age 62,5 ±8,5 years. We included only patients with non-tremor form of PD. Exclusion criteria covered a broad range of conditions and medications that could independently effect on the gut microbiota. All PD patients were using antiparkinsonian medication. PD cohort was performed using Unified PD Rating Scale part II, Non-Motor Symptoms Scale (NMSS), Bristol Stool Form scale, defecation diary. Constipation was defined by the Rome III criteria. Patients were recommended to receive probiotics (Enterococcus (E.) faecium L3, 9 10 ⁸ КОЕ, twice per day) for 2 weeks. Study of the fecal samples of all patients collected before and after receiving probiotic was performed by real time PCR method and bacteriologically.

Results: Gastrointestinal dysfunctions included constipation, malnutrition, dental problems, sialorhea, dysphagia, impaired gastric emptying, defecation difficulties. Constipation was the most prevalent (100%). The duration of constipation was (8.3±5.9) years. Compared with pre-treatment, NMSS scores decreased from 30.1±11.0 to 17.3±5.4 (p<0.01), defecation frequency increased from 1.8±0.6 to 3.7±1.1 per week (p<0.01). The study of fecal samples at the initial stage of the experiment revealed overgrowth of Staphylococcus (S.) aureus, gamma proteobacteri, the evaluation of Bacteroides fragilis (B.f) and Fusobacterium spp. content, decrease of quantity of Fecalibacterium prausnitzii (F.p.) and Bacteroides thetaiotaomicron (B.t.). After administration of probiotic we observed a significant decrease of Fusobacterium spp., Klebsiella spp., Enterobacter spp., S.aureus content; increase in the number of B.t. and F.p. The ratio of B.f. to F.p., as indicator of anaerobic imbalance, decreased (p<0.005).

Conclusions: PD accompanied with the development of intestinal dysbiosis. The introduction of probiotics (E. faecium L3) may contribute to correction of microbiota imbalance and reduce manifestations of dyspepsia and constipation. Further studies may elucidate the temporal and causal relationships between gut microbita and PD and the mechanisms involved in gut brain axis.

References: None

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MDS Abstracts - https://www.mdsabstracts.org/abstract/probiotic-correction-of-intestinal-dysbiosis-in-parkinsons-disease/