Objective: The objective of current investigation was to decipher e the neuroprotective effect of lemon oil (LO) on PC 12 cell lines by 6-hydroxydopamine (6-OHDA) of neuroblastoma of human origin, further to clear the signaling pathways in it.

Background: Its well established fact that Parkinson Disease is a neurodegenerative disease of CNS with progressive nature. No clear etiology is established regarding its pathogenesis, however, investigations suggested that the inflammation and oxidative stress is an important mediator in its pathogenesis.

Method: MTT assay was performed for the estimation of cell viability and also estimation the hydrogen peroxide (H2O2) generation for estimation the intracellular signaling pathways. Furthermore, to determine the mitochondrial membrane potential changes and caspase-3 effect of this compound.

Results: The dose of LO in concentration and time dependent manner treatment of PC12 cells to 6-OHDA (10–1000 μM) down-regulated the cell viability count. The result exhibited that cell death induced via 6-OHDA was mediated through an up-regulation of H2O2 production. It is estimated that there was enhanced caspase 3 activity attributing to depolarization of mitochondrial membrane. LO reduced the reactive oxygen species (42%), nitric oxide (40%), intercellular free Ca2+ (62%) and down-regulated the over production of inducible nitric oxide synthase (51%), nuclear factor κB (56%) and neuronal nitric oxide synthase (28%), respectively. These effects occur due to reduction the production of H2O2 and protect the mitochondrial membrane’s potential and down-regulated of caspase-3 effect.

Conclusion: The result suggests that LO can be promising source with neuroprotective effect via ROS-NO pathway.

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MDS Abstracts - https://www.mdsabstracts.org/abstract/neuroprotective-effect-of-lemon-oil-on-6-ohda-induced-apoptosis-in-pc12-cells-through-the-ros-no-pathway/