Objective: This study aimed to isolate and characterize Olea purpurea leaf extract, and its neuroprotective effect in MPTP induced parkinsonian rat model.

Background: Parkinson’s disease (PD), also known as shaking palsy, is a progressive neurodegenerative disorder that encompasses both motor- and non-motor dysfunctions, with deteriorating effects on mobility and muscle control. Several recent studies have reported the involvement of oxidative stress in neurodegeneration and neuro-inflammation

Method: Olea purpurea leaves were taken, and shade dried. Extract (OPE) was prepared in the soxhlet with the polarity of the solvent. In-vitro antioxidant and total phenol and flavonoid content were assessed. Methanolic leaf extract was found to have maximum antioxidant activity and chosen for further in vivo studies. The extract HPLC analysis showed the presence of flavonoid tyrosol in higher concentrations. A total of 21 rats was taken and divided into three groups. Control, given intraperitoneal injection (i.p) of saline, MPTP intoxicated, given i.p two doses of MPTP (30 mg/kg body weight) at 16 h interval, OPE treated rats, MPTP+OPE (100 mg/kg body weight) orally for 28 days. Bodyweight, locomotor behavior monitored weekly. At the end of the experiment, brains were collected for performing biochemical assays, gene expression, and immunohistochemical analysis.

Results: After seven days of treatment rotarod timing, hanging test was found to decrease, and narrow beam timing increased in MPTP administered rats compared to normal rats. OPE administration improves the timing at normalcy. Brain tissue of MPTP rats shows up-regulated level of lipid peroxidation, nitrite level, protein carbonyl, and down-regulated level of SOD, catalase, and GSH. Restorative effects seen in OPE fed rats. mRNA level analysis showed the elevated level of IL-1β, TNF-α, and downregulation of the Il-10 level in MPTP treated rats while OPE treated rats significantly restored their level. It is apparent from the immunohistochemical study that OPE treatment inhibits the MPTP induced activation of NF-κB.

Conclusion: Our experimental finding suggest that olea purpurea leaf extract showed neuromodulation and neuroprotective potential against MPTP induced nigrostriatal dopaminergic, and also exhibits anti-inflammatory activity.

« Back to MDS Virtual Congress 2020

MDS Abstracts - https://www.mdsabstracts.org/abstract/neuroprotective-and-neuromodulatory-properties-of-olea-purpurea-leaf-extract-in-mptp-intoxicated-parkinsonian-rat-model/