Session Information
Date: Monday, October 8, 2018
Session Title: Parkinson's Disease: Genetics
Session Time: 1:15pm-2:45pm
Location: Hall 3FG
Objective: The aim of this study was to compare the miRNA expression in secreted exosomes from dopaminergic neurons overexpressing α-synuclein with healthy neurons.
Background: Exosomes, nano-sized membrane vesicles, are released into the extracellular environment by most cell types. Recent studies have demonstrated that exosomes are capable of delivering their content (mainly RNAs, proteins, and lipids) to target cells, which is a new way of intercellular communication, removal of cellular debris, and transfer of pathogens between cells. In Parkinson’s disease (PD), exosomes are considered as protective carriers of toxic α-synuclein that contribute to the spread of α-synuclein aggregates in CNS and the dissemination of pathology.
Methods: We first transfected SH-SY5Y cells with either lentivirus expressing wild-type human SNCA or control lentivirus and then collected their exosomes. Microarray was used to detect differential miRNAs of these exosomes. Real-time PCR was used to verify the expression of miRNAs. Target genes of these miRNAs were predicted by TargetScan and miRanda, and then further analyzed by Gene Ontology and KEGG pathway enrichment.
Results: According to the fold change and P value, 10 distinct miRNAs were selected for verification using real-time PCR, and their expression were consistent with the results of microarray. Our analysis revealed the top enriched GO terms of the target genes of differential miRNAs include G-protein coupled photoreceptor activity，voltage-gated ion channel activity involved in regulation of postsynaptic membrane potential，and photoreceptor activity. While TGF-beta signaling pathway, axon guidance, and mTOR signaling pathway are involved in the top enriched KEGG pathways.
Conclusions: In conclusion, the results of the present study showed that exosomes from neurons overexpressing α-synuclein are characterized by a specific miRNA signature, which will have crucial influence on discovery of biomarkers and therapeutic targets for neurodegenerative diseases including PD.
To cite this abstract in AMA style:
Y. Liang, D. Lin, T. Zhou, D. Zheng. Identification and analysis of differential miRNA in exosomes of dopaminergic neurons overexpressing α-synuclein [abstract]. Mov Disord. 2018; 33 (suppl 2). https://www.mdsabstracts.org/abstract/identification-and-analysis-of-differential-mirna-in-exosomes-of-dopaminergic-neurons-overexpressing-%ce%b1-synuclein/. Accessed November 22, 2024.« Back to 2018 International Congress
MDS Abstracts - https://www.mdsabstracts.org/abstract/identification-and-analysis-of-differential-mirna-in-exosomes-of-dopaminergic-neurons-overexpressing-%ce%b1-synuclein/