Objective: Neurodegenerative diseases including Parkinson’s disease (PD) mostly accompany with induction of high ROS in neuronal cells. It is believed that unusual level of ROS may early or consequent reason for degeneration in particular parts of brain.

Background: Rotenone as a pesticide promotes specifically degeneration in the substantia nigra and is widely used to simulate PD model in cells and animals. Furthermore, aggregation of alpha- synuclein (α-SN) has apparently main roles in progression and worsening of PD and other related diseases.

Method: In this study the SHY5Y cells overexpressing α-SN were treated with rotenone (1 µM) / α-SN (70 µM) aggregates in the presence or absence of different concentrations of the methanolic extract of the fruits of some Iranian olive cultivars. The ROS index and also viability of the cells were assessed using DCFH-DA and MTT assays, respectively.

Results: Interestingly all extracts meaningfully suppressed ROS production when co-present with rotenone / α-SN aggregates. On the other hand, viability index indicted that always ROS suppression is not couple with cell protection. In some cases with low ROS we observed a high neurotoxicity; however in other cases the protective effect on the neurotoxicity of rotenone / α-SN aggregates was detected.

Conclusion: It is indicted that neurodegeneration is a high complex phenomena even in a cellular models and needs to increase the knowledge regarding these kinds of diseases.

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MDS Abstracts - https://www.mdsabstracts.org/abstract/high-repressive-activity-of-olive-extracts-on-the-cell-line-model-of-parkinson-disease-against-ros-induction/