Objective: Previous literature has proven that histamine has a crucial role in the process of neuroinflammation in Parkinson’s Disease (PD) . We can indirectly detect histamine levels in fluid samples by quantifying levels of histidine. Therefore, we developed a gold and silver nanoparticle-based LSPR sensor for the detection of histidine. 

Background: The central histaminergic system is one of the subcortical  aminergic  projection  systems  involved  in several  regulatory  functions. It is known that the  central  dopaminergic and  histaminergic  systems  interact in diseases affecting the dopaminergic neurons, one of them being PD. Sensitive determination of specific biomarkers could help the medical community to provide better early diagnosis and disease modifying therapies for patients with Parkinson’s Disease.

Method: Au/Ag and Au, Ag nanoparticles were synthesized. Histidine was prepared at different concentrations: 10 mM, 25mM, and 100 mM. UV-Vis spectroscopy was used to determine the absorbance spectrums of the nanoparticles with histidine. Additionally, a spearman analysis was done for the absorbance values measured from 190 nm to 360 nm in all three concentrations in order to evaluate the significance of the study and to observe correlations between the variables.

Results: In all concentrations a higher absorbance was obtained with the metallic systems. At 10 mM both Au and Ag generated a better signal than histidine alone, at a wavelength of 217 nm with an absorbance of 0.76 at 25 mM, Au emitted the highest signal at a wavelength of 221 with an absorbance of 0.39 and at 100 mM the system Au/Ag generated a better signal at a wavelength of 209.0 at an absorbance of 0.89. Additionally, the conducted spearman analysis indicated p < 0.001 in all concentrations.

Conclusion: We successfully developed an LSPR sensor that improved the detection of histidine. Our findings could help to easily identify abnormal levels of relevant biomarkers in PD and contribute to early detection of this disease.

References: references

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MDS Abstracts - https://www.mdsabstracts.org/abstract/gold-and-silver-nanoparticle-based-localized-surface-plasmon-resonance-sensor-lspr-for-the-detection-of-histidine-as-a-potential-biomarker-in-parkinsons-disease/