Objective: In the present work, we investigated the protective effects of ginsenoside Rb1 in dopaminergic neurons through Foxm1-Nurr1 pathway in mice model of Parkinson’s disease.

Background: Parkinson’s disease(PD) is the second most common neurodegenerative disease which is caused by progressive degeneration of dopaminergic neurons in the substantia nigra pars compacta(SNpc). The underlying mechanisms of the PD has not been fully understood. Foxm1 is a transcription factor of proliferation. Foxm1 could bound to the promoter region of the Nurr1 which is important in development and maintenance of dopaminergic neurons.

Methods: C57BL/6J mice were used to administerd MPTP(30mg/kg) toxin to establish the PD mice model and treated with Rb1(5 mg/kg, 10 mg/kg, 20 mg/kg). “Pole test” was used to test the motor coordination of mice. The dopaminergic cells in the SNpc region were measured with tyrosine hydroxylase-positive immunofluorescent staining. TUNEL was applied to measure the apoptosis of cells in SNpc region. Protein and mRNA expression of Foxm1 and Nurr1 were determined with western blot and Reversed Transcript Polymerase Chain Reaction.

Results: Rb1 treatment ameliorated the behaviour injury and dopaminergic cells loss induced by MPTP in a dose-dependent manner in mice model of PD. The cell apoptosis of the SNpc region was reduced with the Rb1 compared with the MPTP group. Protein and mRNA expression of Foxm1 and Nurr1 was induced with Rb1 treatment compared with the MPTP group.

Conclusions: Our results provide the evidence that Rb1 has neuroprotective effects via Foxm1-Nurr1 pathway in mice model of Parkinson’s disease.

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MDS Abstracts - https://www.mdsabstracts.org/abstract/ginsenoside-rb1-protects-dopaminergic-neurons-through-foxm1-nurr1-pathway-in-mice-model-of-parkinsons-disease/