Objective: The present study was aimed to explore the synergistic neuroprotective effect of combination of natural phenolic compound, i. e., chlorogenic acid, and butanol extract of Trianthema portulacastrum (CA+ BuTP) against rotenone induced Parkinson’s disease (PD) rat model via behavioral, oxidative stress, neurotransmitter and mitochondrial hypofunction estimation.

Background: Chlorogenic acid has potential neuroprotective effect against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine mouse model of Parkinson’s disease (PD) by correcting the tyrosine hydroxylase -positive dopaminergic neurons in the substantia nigra pars compacta and striatal neurotransmitters level along with locomotor activity. Traditionally, Trianthema portulacastrum (TP) leaves utilized to improve wandering of mind. Previous reports exhibited that TP has antioxidant, hepatoprotective and anti-Alzheimer activity.

Method: Animals were randomly divided into five groups, i. e., Group I and II were treated with saline and rotenone (1 mg/kg, i.p.), respectively, whereas Group III- V were administered with (CA+ BuTP) combination at three different dose levels 100, 200 and 400 mg/kg, p.o. as well as injected with 1 mg/kg, i.p. rotenone for 21 days consecutively. A significant cognition and locomotor deficit was observed in rotenone group as compared to control group. Furthermore, enzymatic antioxidant status including superoxide dismutase (SOD), catalase (CAT) and glutathione peroxide level (GPx) as well as acetylcholinesterase (AChE) and dopamine levels were also reduced. Decrease in Complex-II (succinate dehydrogenase) and Complex-III (3- (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) enzymatic activity within rotenone treated rats brain signified the mitochondrial hypofunction.

Results: CA+BuTP preadministration significantly attenuates (p< 0.05) damaging effects dose-dependently as compared to rotenone-treated group.

Conclusion: CA+BuTP combination approach showed synergistic and neuroprotective propensity against rotenone induced Parkinsonism in rat model. Therefore, it may be utilized as a potential therapeutic approach for management of PD.

« Back to 2019 International Congress

MDS Abstracts - https://www.mdsabstracts.org/abstract/chlorogenic-acid-and-trianthema-portulacastrum-combinational-therapy-mitigates-rotenone-induced-parkinsonism-in-wistar-rats/