Objective: We aimed to evaluate cerebrospinal fluid (CSF) levels of oxidative stress (OS) and antioxidant potential using diacron-reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) in Parkinson’s disease (PD) and progressive supranuclear palsy (PSP) in this study.

Background: OS has been noticed and investigated as one of the candidates of the pathogenesis of PD. Until now, evaluating OS and antioxidants agents has been complicated and difficult to perform clinically. D-ROMs and BAP are new handy methods to evaluate overall OS and antioxidant potential, based on the Fenton’s reaction. Several studies have used these two tests to evaluate plasma levels of OS and antioxidant potential. However, blood samples can be influenced by systemic clinical conditions such as infection and metabolic disorders. CSF is regarded as better samples reflecting central nervous system levels of OS and antioxidant potential, but there is no study measuring OS and antioxidant potential in CSF samples of patients with PD.

Method: CSF samples were obtained from 69 patients with PD, 14 patients with PSP, and 22 normal controls (NC). CSF levels of OS and antioxidant potential were measured using d-ROMs and BAP, respectively. The results were compared with the clinical backgrounds among PD, PSP and NC.

Results: CSF levels of d-ROMs in all groups were significantly lower (˂10 U.CARR) than those observed in the plasma levels of healthy adults (approximately 250-300 U.CARR). CSF levels of antioxidant potential were significantly higher in patients with PSP (175 ± 79 μM) than in patients with PD (918 ± 350 μM) (p = 0.019). In PD group, antioxidant potential was significantly lower in patients with tremor (858 ± 269 μM) than in those without tremor (1132 ± 505 μM) (p = 0.004).

Conclusion: Our study suggests that CSF levels of OS are under homeostatic control of antioxidative mechanisms in healthy conditions and neurodegenerative diseases as well, and increased antioxidant potential can indicate the CSF levels of OS. The lower CSF levels of OS may associate with the benign phenotype in the tremor dominant PD. Difference of CSF antioxidant potential levels between PD and PSP may associate with the background pathology which can be useful for clinical diagnosis of these two diseases.

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MDS Abstracts - https://www.mdsabstracts.org/abstract/cerebrospinal-fluid-levels-of-oxidative-stress-measured-using-diacron-reactive-oxygen-metabolites-and-biological-antioxidant-potential-in-patients-with-parkinsons-disease-and-progressive-supr/