Objective: This study explores the interplay encompassing amino acids’ roles, metabolic processes, growth, and the intricate relationships between sleep patterns, amino acid-deficient diet, and caffeine consumption, with emphasis on the vital connections between diet, oxidative stress, and cognitive health while investigating the essential role of quality sleep in maintaining cognitive function.

Background: Emphasizing the crucial links between diet, oxidative stress, and cognitive health, the research underscores the essential role of quality sleep in maintaining cognitive function. The bidirectional relationship between disrupted sleep and cognitive health, as well as the impact of caffeine on cognitive function, is explored.

Method: Adult male Wistar rats, divided into groups A and B, are subjected to different conditions. Group A, in a standard environment, receives diets with varying tryptophan levels, while Group B undergoes sleep deprivation with varying tryptophan levels, including caffeine. The three-week experiment involves blood sample collection, biochemical assays, and brain histology.

Results: Results reveal a linear decline in melatonin and serotonin levels in caffeine-administered groups, potentially linked to stress. Sleep-deprived rats exhibit elevated biomarkers during sub-chronic sleep deprivation, proposing melatonin and serotonin as potential sleep deprivation indicators. Testosterone and insulin-like growth hormone assays indicate a substantial decrease, impacting secondary sex characteristics and growth. Elevated TNF-α and interleukin-1β levels suggest health risks, while antioxidant enzyme assays indicate heightened oxidative stress. Histopathological studies reveal cellular abnormalities, hinting at increased metabolism and potential pathologies in sleep-deprived conditions.

Conclusion: The study underscores caffeine’s influence on biochemical parameters, specifically oxidative stress during sleep deprivation, providing nuanced insights into the intricate interplay between sleep disruption, caffeine, and biochemical markers. This research contributes to an enhanced understanding of the physiological and biochemical consequences of sleep disruption, offering valuable perspectives for future studies and potential advancements in clinical and preventive approaches in the coming years.

References: [1] Alkadhi, K., Zagaar, M., Alhaider, I., Salim, S., and Aleisa, A. (2013). Neurobiological consequences of sleep deprivation. Current neuropharmacology, 11(3), 231-249

[2] Lesku, J. A., Rattenborg, N. C., and Amlaner, C. J. (2016). The evolution of sleep: a phylogenetic approach. Sleep: A comprehensive handbook, 1, 1038.

[3] Van Dongen, H., Maislin, G., Mullington, J. M., and Dinges, D. F. (2003). The cumulative cost of additional wakefulness: dose-response effects on neurobehavioral functions and sleep physiology from chronic sleep restriction and total sleep deprivation. Sleep, 26(2), 117-126

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MDS Abstracts - https://www.mdsabstracts.org/abstract/assessment-of-the-effect-of-sleep-deprivation-and-essential-amino-acid-deficient-diet-tryptophan-on-growth-and-cognition-in-wistar-rats/