Session Information
Date: Tuesday, June 21, 2016
Session Title: Parkinson's disease: Genetics
Session Time: 12:30pm-2:00pm
Location: Exhibit Hall located in Hall B, Level 2
Objective: To examine SNCA mRNA levels in CD45+ peripheral blood cells in patients with dementia with Lewy bodies (DLB), Parkinson’s disease (PD) and individuals without neurological disorders.
Background: Clinical and neuropathological similarities between DLB and PD suggest that these disorders may share etiology. Last data of biochemical and genetic evidence showed the formation of alpha-synuclein (SNCA) protein deposits is an important step in the development of these diseases. Existed criteria cannot reliably differentiate between the two clinical entities.
Methods: 16 DLB patients (9 females, 7 males, mean age 64.1±10.7), 43 PD patients (25 females, 18 males, mean age 60.7±8.4) and 39 individuals without neurological disorders (controls) (25 females, 14 males, mean age 62.1±9.5) were included in this study. SNCA mRNA levels in CD45+ were estimated by quantitative RT-PCR PCR with iTaq Universal SYBR Green Supermix (Bio-Rad, USA). GNB2L1 mRNA levels were used as internal control. CD45+ were selected by MACS system (magnetic cell sorting system) (Miltenyi Biotec, USA).
Results: In comparison with controls, SNCA mRNA levels in DLB patients were significantly higher (p=0,006). However, SNCA mRNA levels did not differ among PD patients and controls (p = 0.952). Interestingly, SNCA mRNA level in DLB patients were elevated compared to PD patients (p = 0.006). Median values relative SNCA expression were 1.59 (0.36-9.87) in DLB patients, 0.38 (0.01-7.33), in PD patients and 0.41 (0.01-6.67) in controls.
Conclusions: This is the first report about higher SNCA expression in CD45+ in DLB patients compared to PD patients and controls. Our results suggest that SNCA mRNA levels might help in the differentiation of DLB from PD.
To cite this abstract in AMA style:
N. Nikolaev, B. Lu.A., P.A. Andoskin, A. Emelyanov, A.E. Kopytova, K.A. Senkevich, I.V. Milyukhina, S.N. Pchelina. Elevated SNCA expression in CD45+ peripheral blood cells in patients with dementia with Lewy bodies [abstract]. Mov Disord. 2016; 31 (suppl 2). https://www.mdsabstracts.org/abstract/elevated-snca-expression-in-cd45-peripheral-blood-cells-in-patients-with-dementia-with-lewy-bodies/. Accessed November 22, 2024.« Back to 2016 International Congress
MDS Abstracts - https://www.mdsabstracts.org/abstract/elevated-snca-expression-in-cd45-peripheral-blood-cells-in-patients-with-dementia-with-lewy-bodies/