Objective: Allicin is flavonoid that possess neuroprotective potential but main impediment is its low bioavailability which was improved with microemulsion. The present study was framed to find the neuroprotective effect of allicin microemulsion against rotenone and manganese induced Parkinsonism in rats.

Background: Rotenone is a neurotoxin that inhibits mitochondrial ETC complex-I and causes dopaminergic neurodegeneration, deficiency of dopamine in brain accompanied by Parkinsonism both in human and rodents. Manganese is a trace element required for the normal growth and development in human and animals but at higher concentration it causes CNS dysfunction known as manganism also synergies the toxic effect of rotenone.

Method: Rats were administered with rotenone (6µg/µl/kg) once unilaterally and manganese (30mg/kg; p.o.) were given for 28 days. Behavioral (open field, rotarod, narrow beam walk, grip strength) analysis was performed weekly on 1^st 7^th 14^th 21^st and 28^th day. After rotenone and manganese, on 7^th day animals were administered with allicin microemulsion (10 and 30 mg/kg; i.p.) for 21 days. Rats were sacrificed on day 29^th and homogenate of brain (striatal) tissues were taken for biochemical (MDA, GSH and nitrite), neuroinflammatory cytokines ( IL-1β and TNF-α) and neurotransmitters (dopamine, GABA, glutamate) analysis.

Results: Rotenone and manganese administration drastically altered motor co-ordination, induced biochemical, Neuroinflammatory and neurotransmitter changes. Allicin microemulsion (10 and 30 mg/kg; i.p.) significantly alleviated the rotenone and manganese induced motor deficit, biochemical and neurotransmitters alteration in rats through antioxidant, anti-inflammatory and neuroprotective mechanisms.

Conclusion: Present observation of the study revealed that allicin microemulsion could serve as fascinating strategy in preventing neurodegeneration through antioxidant, anti-inflammatory and neuromodulatory potential.

References: Donzanti BA, Yamamoto BK (1988) An improved and rapid HPLC-EC method for the isocratic separation of amino acid neurotransmitters from brain tissue and microdialysis perfusates. Life sciences 43,913-922. Liu TH, Luo CL, Huang B, Lu TS and Fu YS (2018) The caffeine effects on rotenone-induced Parkinson’s disease model in vitro and in vivo. The FASEB Journal, 32, pp.740-2.

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MDS Abstracts - https://www.mdsabstracts.org/abstract/neuroprotective-effects-of-allicin-microemulsion-against-intrastriatal-rotenone-and-manganese-induced-parkinsonism-in-rats/