Objective: We tried to study the effect of Norepinepherine and Serotonin on  Sodium Potassium ATPase in rat brain synaptosome.

Background: Sleep and wakefulness are behavioral and physiological activities. Sleep has been defined as a reversible behavioral state of perceptual disengagement from and unresponsiveness to the environment.

Method: We have studied the effect of Norepinepherine, Serotonin (5HT) individually, in combination and in presence of receptor antagonists Prazosin and Propranolol in different permutations and combinations.

Results: It was found that Norepinepherine increases Na+/K+ ATPase activity. It was also validated that NE induced increase is blocked by prazosin which is a α-1 adrenoceptor blocker antagonist but propranolol which is a β adrenoceptor blocker antagonist cannot block it. There is a marginal but significant increase in Na+/K+ ATPase activity. Propranolol seems to block 5HT mediated increase in Na+/K+ ATPase activity. When both NE and 5HT are given in combination an increase in ATPase activity has been found which is equal to the sum of the two given separately. Both Prazosin and Propranolol could decrease the basal values of the enzyme which could be due to some mechanism involving endogenous NE and 5HT present in the sample or by other mechanism leading to nonspecific binding and decrease of ATPase activity.

Conclusion: Many sleep disorders could be addressed by studying the significance of NE  and 5HT with blockers.

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MDS Abstracts - https://www.mdsabstracts.org/abstract/modulation-of-na-k-atpase-activity-of-rat-brain-synaptosome-by-norepinepherine-and-serotonin/