Objective: To study the changes of the expression of silent information adjustment factor 1 (SIRT1) and hypoxia inducing factor 1 (HIF-1) and changes in behavior in mice of MPTP treatment.

Background: Silent information adjustment factor 1 (SIRT1) and hypoxia inducing factor 1 (HIF1) were found to involve in the pathogenesis of Parkinson’s disease and plays an important role in disease progression.

Methods: To establish animal model of PD, MPTP was chose to treat C57BL6 mice, and then behavioral tests, HPLC and immunohistochemistry were conducted. Further, the levels of SIRT1 and HIF1- was detected.

Results: Mice in MPTP-treated group displayed a significant abnormal behavior , autonomic activity, gait and bradykinesia (all P<0．001). HPLC, which was used to investigate the levels of neurotransmitters, revealed decreased levels of DA and its metabolites (P<0．001) in MPTP-induced mice. MPTP reduced the levels of tyrosine hydroxylase (TH) and the dopamine transporter (DAT) remarkably (P<0．01), which are markers of substantia nigra dopaminergic neurons. At last, the expression levels of SIRT1 (P<0．05）was reduced and HIF1 (P<0．05） was increased in MPTP group compared with control group.

Conclusions: Abnormal behavior and decline of dopaminergic neurons markers prompted the successful establishment of PD model. The irregular expression of SIRT1/HIF – 1 in vivo supported our previous studies, and indicated that SIRT1 / HIF1 signaling may involve the disease process of PD.

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MDS Abstracts - https://www.mdsabstracts.org/abstract/the-change-of-sirt1-and-hif-1-in-mptp-treated-mouse/