Objective: This study aims to quantify and correlate Mortalin and α-Syn protein in serum of Parkinson’s disease patients and develop it as protein marker for early detection of Parkinson’s disease.

Background: Mortalin is associated with Parkinson’s disease (PD) both physiological as well as clinical. Mortalin, a mitochondrial chaperone, plays significant role in reducing toxicity of lewy bodies. The earlier study reported lower expression of Mortalin in different models of PD patients. In PD, increased level of Mortalin suppresses the toxicity of aggregated α-Syn (1)

Methods: The expression of Mortalin and α-Syn in serum samples of 38 PD patients and 33 control group (CG) individuals were estimated by surface plasmon resonance (SPR). The receiver operating characteristic (ROC) curves were constructed to develop it as blood based protein marker. The level of Mortalin in serum was confirmed by western blot.

Results: The Mortalin level was found to be downregulated in PD patients (1.98±0.53 ng/µL) in comparison to CG individuals (3.13±0.48 ng/µL) whereas α-Syn level was found to be upregulated in PD patients (38.20±4.22ng/µL) than CG individuals (34.31±3.23 ng/µL) in serum. The negative correlation between Mortalin and α-Syn was revealed by statistical analysis

Conclusions: This preliminary study concluded that Mortalin plays a crucial role in PD with negative correlation with α-Syn. This study provides a new platform for the development of Mortalin as a potent serum protein diagnostic marker for PD.

References: 1. Zhu J., Vereshchagina N., Sreekumar V., Barbulla L.F., Costa A.C., Daub K.J., Woitalla D., Martis L.M., Kruger R., Rasse T.M., 2013. Knockdown of Hsc70-5/mortalin Induces Loss of Synaptic Mitochondria in a Drosophila Parkinson’s Dis-ease Model. PLoS ONE 8, e83714.

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MDS Abstracts - https://www.mdsabstracts.org/abstract/level-of-serum-mortalin-in-parkinsons-disease-correlating-with-%ef%81%a1-synuclein-assessment-of-protein-marker-for-early-detection/