Objective: Impact of psychological stressors in modulating the toxicity of environmental chemicals has been suggested, however the mechanism this is not clear.

Background: Repeated stress exaggerates the toxicity of environmental chemicals/drugs including metals and pesticides have been reported in number of experimental studies although the correct mechanism associated with is not clearly understood. The present study has been carried out to investigate the consequence of immobilization stress (IMS), a psychological stressor on the neurobehavioral toxicity of lambda-cyhalothrin (LCT), a new generation type-II synthetic pyrethroid with imminent uses to control insects, pest and vector born disease in public health programmes.

Methods: Rats were encountered with IMS (placed in plastic restrainer for 15 min/day; one session/day) for 28 days or exposed with LCT (3.0 mg/kg body weight, p.o.) for 3 days (on days 26, 27 and 28) or pre-exposed to IMS for 28 days followed by LCT treatment for 3 days. Plasma corticosterone, blood brain barrier (BBB) permeability and inflammatory cytokines in corpus striatum were estimated by standard procedure. Further, effect of dopamine signaling associated with motor functions was also assessed by standard protocol.

Results: Increased the levels of plasma corticosterone and alteration of blood brain barrier permeability was found in rats pre-exposure to IMS followed by LCT treatment as compared to IMS or LCT alone. Additionally, decreased the expression of dopamine signaling and neuroinflammatory cytokines in corpus striatum have also been observed in these rats as compared to rats exposed to either IMS or treated with LCT alone. These changes coupled with motor dysfunctions. Marginal changes was observed in dopamine signaling and inflammatory cytokines in corpus striatum including plasma corticosterone levels and blood brain barrier permeability associated with motor functions in rats exposed to either IMS or treated with LCT alone as compared to controls.

Conclusions: The results revealed that repeated stress considerably modulates the neurotoxicity of LCT through neuroinflammatory cytokines associated with dopaminergic singling which regulate the movement disorders.

References: 1. Rajendra K Shukla, Yogesh Dhuriya, Lalit P Chandravanshi, Richa Gupta, Pranay Srivastava, Aditya B Pant, Ajay Kumar, M. Haris Siddiqui and Vinay K Khanna. Influence of immobilization and forced swim stress on the neurotoxicity of lambda-cyhalothrin in rats: Effect on brain biogenic amines and BBB permeability. Neurotoxicology 60 (2017) 187 – 196. 2. Rajendra K Shukla, Richa Gupta, Yogesh Dhuriya, Lalit P Chandravanshi, Pranay Srivastava, Aditya B Pant, Ajay Kumar, M. Haris Siddiqui and Vinay K Khanna. Brain cholinergic alterations in rats subjected to repeated immobilization or forced swim stress on lambda-cyhalothrin exposure. Neurochemistry International. 93 (2016) 51–63.

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MDS Abstracts - https://www.mdsabstracts.org/abstract/dopaminergic-impairments-following-repeated-exposure-to-stress-on-the-neurotoxicity-of-lambda-cyhalothrin-through-inflammatory-cytokines-in-rats/