Objective: This study examined the effect of pretreatment with Myristicin on the severity of 6-hydroxydopamine-induced Parkinsonism in Wistar rats in order to find out whether Myristicin could attenuate oxidative stress markers, behavioral and structural abnormalities in an experimental model of PD in rat.

Background: Parkinson’s disease refers to a progressive neurodegenerative disorder involving degeneration of dopaminergic neurons particularly in substantia nigra. The loss of dopaminergic cells results in complex motor syndrome. One of the hypothesis in pathogenesis of PD is oxidative stress via NADPH-dependent oxidases. Myristicin have been reported to increase oxidative burden.

Methods: 72 male wistar rats were divided into six groups: (1) sham, normal saline was injected in the left SNC (substantia nigra pars compacta) , (2) 6-OHDA, 6-hydroxydopamine was injected into left SNC, (3) 6-OHDA+Captopril (5 mg/kg, i.p), (4) 6-OHDA+Myristicin (5 mg/kg, i.p), (5) 6-OHDA+Myristicin (20 mg/kg, i.p), (6) 6-OHDA+Myristicin (100 mg/kg, i.p). Myristicin and captopril were injected rats daily from six dayes before the surgery to a day after. At the end of the experiment brain’s oxidative stress markers; Lipid peroxidation and protein oxidation, apomorphine-induced rotational asymmetry were measured and the number of Nissl-stained neurons in SNC after 2 weeks were counted.

Results: Myristicin administration could attenuate the rotational behavior (p﹤0,05). and Myristicin also decrease the amounts of oxidative stress markers in lesioned rats and protect the neurons of SNC against 6-OHDA toxicity in comparison with the group which received only neurotoxin (p﹤0,01).

Conclusions: In conclusion, the results of the present study suggest that Myristicin administration has a protective effect against 6-OHDA toxicity and may be useful for treatment of parkinson’s disease.

References: Moradganjeh, A. 2013. Losartan pretreatment reduces neurodegeneration … – Pathophysiology. http://www.pathophysiologyjournal.com/article/S0928-4680(13)00055-2/abstract. Zhang, H. 2006. Evaluation of antioxidant activity of parsley (Petroselinum crispum … http://www.xoc.uam.mx/files/4958/application/pdf/Evaluation-of-antioxidant-activity-of-parsley-(Petroselinum-crispum)-essential-oil.pdf.

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MDS Abstracts - https://www.mdsabstracts.org/abstract/protective-effect-of-myristicin-against-neurodegeneration-in-a-6-hydroxydopamine-induced-model-of-parkinsons-disease-in-rats/