Session Information
Date: Tuesday, June 6, 2017
Session Title: Therapy in Movement Disorders
Session Time: 1:45pm-3:15pm
Location: Exhibit Hall C
Objective: To assess the effects of autologous implantation of fibroblasts in the internal globus pallidus of dyskinetic MPTP-lesioned monkeys upon motor behaviour and L-DOPA response.
Background: Autologous Implantation of fibroblasts (AIF) in the entopeduncular nucleus of 6-hydroxydopamine-treated rats inhibits L-dopa-induced dyskinesia (LID), and unilateral AIF to internal globus pallidus (GPi) of MPTP-induced parkinsonian macaques reduces LID on the contralateral side. We now present the effect of bilateral AIF to GPi on LID and parkinsonian behavior of MPTP-induced parkinsonian macaques.
Methods: Seventeen macaques were divided into 3 groups; control (n=5), therapeutic dose of fibroblasts as determined in previous experiment (n=6) and double this dose of fibroblasts (n=6). Parkinsonism was induced by daily MPTP administration until the animals attained a score of 7 on an accepted parkinsonism scale for MPTP-treated non-human primates. Daily treatment with Madopar (50% of the optimal dose for suppression of parkinsonism) was commenced until dyskinesia developed and became stable. Autologous fibroblasts or medium (control) were administered bilaterally to GPi.
Results: In months 1-7, both doses of fibroblasts increased the locomotor response to Madopar but enhanced dyskinesia. In months 8-9, a 25% dose of Madopar reduced parkinsonian symptoms without causing dyskinesia, and increased good on-time (GOT). In month 11, in addition to Madopar, pramipexole was administered at 100% and 33% optimal dose, which also improved parkinsonian symptomatology without causing dyskinesia and with increased GOT (Fig. 1).
Conclusions: Fibroblast implantation potentiates good effects of L-dopa and enables use of a lower, non-dyskinetic, dose.
To cite this abstract in AMA style:
E. Bezard, J. Finberg, W.K. Ko, Q. Li, B. Dehay, E. Pioli, Y. Yair. Fibroblasts Implanted in Internal Pallidum of Parkinsonian Macaques Exert L-Dopa-Sparing Activity [abstract]. Mov Disord. 2017; 32 (suppl 2). https://www.mdsabstracts.org/abstract/fibroblasts-implanted-in-internal-pallidum-of-parkinsonian-macaques-exert-l-dopa-sparing-activity/. Accessed September 7, 2024.« Back to 2017 International Congress
MDS Abstracts - https://www.mdsabstracts.org/abstract/fibroblasts-implanted-in-internal-pallidum-of-parkinsonian-macaques-exert-l-dopa-sparing-activity/