Objective: Parkinson’s disease (PD) induced by the interaction between the number of factors viz., aging, genetics toxins, mitochondrial deformity and oxidative stress. Various evidences suggest that regular consumption of an antioxidant rich food may alter the incidence of neurodegenerative diseases. The aim of the current study was to prepare and scrutinize the nanoherbaceutical formulation containing crocetin against 6-hidroxidopamine (6-OHDA) induced PD animal model.

Background: PD is the most prevalent neurodegenerative disorder after Alzheimer’s disease. The loss of dopamenergic neurons in brain results in the lower level of dopamine in the brain regions like Subtantia nigra which ultimately leads to the development of PD.

Methods: Top-down technique was used for the preparation of nanoherbaceutical suspension. Intracerebroventricular injection of 6-OHDA was used for induction the PD and mice were treated with the nano-formulation of crocetin for 28 days. The enzymatic activities viz., Catalase (CAT), superoxide dismutase (SOD), glutathione (GSH), glutathione S-transferase (GST), glutathione peroxidase (GPx) and level of reactive oxygen species (ROS), total reactive antioxidant dopamine and its metabolites homovanillic and 3,4-dihydroxyphenylacetic acid, were scrutinized in the striatum. Behavioural parameters including locomotor, memory and depressive were estimated, respectively.  

Results: The result clearly showed that nano-formulation crocetin treatment was efficient in averting the memory destruction in Morris water maze test as well as depressive like behaviour in tail suspension test. Nano-formulation crocetin attenuated 6-OHDA induced down-regulation in total reactive antioxidant, CAT and GPx, dopamine and its metabolite level in striatum of mice. We also observed the up-regulation levels of GR and reactive oxygen species in the striatum and these modulations were diminished by nano-formulation of crocetin.  

Conclusions: Collectively, we can concluded that nanoherbaceutical formulation of crocetin showed a protective effect on 6-OHDA induced neurotoxicity in mice, signifying that it could be alternative therapy for the treatment of PD.

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MDS Abstracts - https://www.mdsabstracts.org/abstract/development-of-nano-formulation-containing-crocetin-for-the-protective-and-beneficial-effect-against-6-hydroxydopamine-induced-parkinsons-disease-model-via-altered-the-genetic-backgrounds/