Objective: In this study, we evaluated the concentrations of sesn1 and sesn2 in the serum of 50 PD patients and 60 elderly controls. Aging is a major risk factor for Parkinson’s disease (PD). 

Background: PD is characterized by loss of dopaminergic neurons of substantia nigra and presence of cytoplasmic inclusions (Lewy bodies).The muscular rigidity and atrophy with bradykinesia is common in PD. Sestrins (sesn), an antiaging protein, plays an important role in aging related diseases, owing to their antioxidative capacity, which prevents reactive oxygen species (ROS) formation and through regulation of pathways like AMPK-mTOR. Sestrins are shown to be key inducers of autophagy, which is generally considered to be beneficial for preventing age associated disease like PD

Methods: The concentration sestrin proteins were evaluated by using surface plasmon resonance. The serum sestrin levels were further compared using western blot. 

Results: The results showed significant decline (P<0.0001) in the levels of sesn1 protein in the sera of PD group (95% CI: 8.05-8.70 ng/μl) as compared to control group (95% CI: 12.36-14.51 ng/μl). However, an elevated level of sesn2 (P<0.0001) was found in the sera of PD group (95% CI: 15.52-17.11 ng/μl) but not in control group (95% CI: 13.28-14.37 ng/μl). A highly sensitive, selective cutoff values for sesn1 (9.41 ng/μl) and sesn2 (14.90 ng/μl) was determined after the construction of ROC curve to differentiate PD from controls. 

Conclusions: This study opens up the important insights about the role of sestrins in the progression of PD and may help to establish sestrins as potential candidate for protein marker in detection of PD.

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MDS Abstracts - https://www.mdsabstracts.org/abstract/evaluation-of-serum-sestrin-protein-in-parkinsons-disease-a-plausible-diagnostic-marker/